What Does ceesdekker.net Mean?
What Does ceesdekker.net Mean?
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‘The second is nanopores, which can be our oldest line of investigation and that has now led to DNA sequencers depending on nanopores. We pull particular person DNA strands via a nanopore and might then read through off the nucleotide sequence. Really just lately, we even managed to read through out an individual protein for the first time utilizing this technique. But this line of investigate is not only about acquiring instruments, but in addition about actual biological queries.
It had been only in the nineties nevertheless, when nanotechnology had Highly developed into a amount in which one molecules could possibly be manipulated, that the main experimental gadgets commenced to look.
Multicolor imaging of your bacterial nucleoid and mobile division proteins with blue, orange, and around-infrared fluorescent proteins
Considering the fact that 2000, Dekker has shifted the key concentration of his function to biophysics exactly where he reports the Attributes of single biomolecules and cells using the equipment of nanotechnology. This alteration of area was driven by his fascination for that extraordinary functioning of biological molecular constructions, and also via the lengthy-phrase point of view that numerous interesting discoveries might be predicted During this field.[5] Present lines of analysis in his biophysics group are while in the regions of:[six]
2012, discovery that nucleoid occlusion underlies the precision of bacterial cell division; and first at any time study of your dynamics DNA supercoils and the discovery of supercoil hopping
Experimental period diagram of negatively supercoiled DNA measured by magnetic tweezers and fluorescence,
What distinguishes biology from chemistry is that these elements together make up a residing system. The trick of reported daily life, I think, lies from the interactions, the interplay of These molecules. The large query concerning the origin of daily life continues to be unanswered. How did we at any time evolve from basic molecules into the really complex programs we see today? We don't know. I need to operate on these kinds of massive, open up concerns.’
Dynamic ParB–DNA interactions initiate and keep a partition condensate for bacterial chromosome segregation
‘Indeed, I have an understanding of folks were being shocked at enough time. At the end of the nineties, Once i was in my forties, I had been Professor of Molecular Electronics and I have to admit that it experienced turn into a bit bored, I didn’t understand A lot any more. The sphere went more into the appliance facet, how you could integrate nanotubes into Digital circuits.
‘The fantastically refined way through which aspects in character operate by no means ceases to amaze me. If we solve the puzzle of how everyday living came into staying, I hope that my amazement in the complexity of creation can have elevated and with it my awe with the greatness with the Creator.’
Since 2000 he moved to solitary-molecule biophysics and nanobiology, with study from studies of DNA loop extrusion and supercoiling to DNA translocation by nanopores. A lot more recently his ceesdekker exploration has focused on finding out chromatin structure and mobile division with bacteria on chip, when he is additionally seeking to in the end Establish synthetic cells from The underside up.
Nanoscientist Cees Dekker is an amiable and flamboyant person who seems Considerably more youthful compared to 62 decades he essentially is – stylish glasses, psychedelic shirt, partaking smile – although not a single to waste time. He usually takes his time, but not more than required.
For instance, we look at the nucleopore elaborate, a gateway involving the cell nucleus and the cytoplasm. This is a really challenging program and therefore tricky to examine within a mobile, so we use biomimetics. We reconstruct holes and afterwards insert personal elements with the nucleopore intricate to study transportation via it at The one-molecule degree.’
It was an incredibly interesting time, in the course of which I gained a lot of help from Delft College of Technology, in which I used to be a professor at enough time, And that i started numerous collaborations with molecular biologists. That aided me master a lot.’